Biophotonics Center at Vanderbilt University, Keck FEL Center, Suite 200, 410 24th Ave. South, Nashville, TN 37232
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Design and construction of a light-sheet microscope (LSM) for rapid in-vivo volumetric imaging. Optical sectioning is an inherent challenge for volumetric imaging, due to traditional strategies (like confocal or other laser-scanning techniques) being too slow to image dynamic processes, as well as causing photobleaching that alters sample biochemistry. Scanned oblique plane illumination (SOPi) is a single-objective implementation of LSM that is sterically accessible for imaging any sized sample, and performs optical sectioning that is undistorted during scanning of the light-sheet. The utility of this SOPi microscopy platform will be realized through collaborative work within and outside of the Biophotonics Center.