Spectrophotometry

Tutorial Quiz

Write the answer to the following questions on the answer sheet found in your laboratory manual and turn it in at the beginning of the laboratory period as instructed by your TA.

What would be the molar absorptivity of a substance that had an absorbance of 0.119 and a concentration of 9.9 x 10-4M?  The cell path length used to measure this substance was 1.0 cm.

 

Describe the procedure you would use to determine the concentration of a light absorbing molecule using spectrophotometric methods.

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