pH6Ulp1

For expression of N-terminally His-tagged Ulp1 (Sumo protease) in E. coli.

General Information:

• pET derivative
• T7lac promoter
• low copy
• Kan resistance
• dna sequence
• Encodes catalytic domain of S. cerevisiae Ulp1, residues 430-621
• MW = 26.8 kDa
• Amino acid sequence:

MGSSHHHHHH GGGLVPELNE KDDDQVQKAL ASRENTQLMN RDNIEITVRD FKTLAPRRWL NDTIIEFFMK YIEKSTPNTV AFNSFFYTNL SERGYQGVRR WMKRKKTQID KLDKIFTPIN LNQSHWALGI IDLKKKTIGY VDSLSNGPNA MSFAILTDLQ KYVMEESKHT IGEDFDLIHL DCPQQPNGYD CGIYVCMNTL YGSADAPLDF DYKDAIRMRR FIAHLILTDA LK

Protocol: Ulp1 expression and purification