pH6H3C

For expression of N-terminally His-tagged human rhinovirus 3C protease in E. coli.

General Information:

• pET derivative
• T7lac promoter
• low copy
• Kan resistance
• dna sequence
• 3C protease recognition sequence: LEVLFQ↓GP
• MW = 21.8 kDa
• Amino acid sequence:

MGSSHHHHHH GGGLVPRGPN TEFALSLLRK NIMTITTSKG EFTGLGIHDR VCVIPTHAQP GDDVLVNGQK IRVKDKYKLV DPENINLELT VLTLDRNEKF RDIRGFISED LEGVDATLVV HSNNFTNTIL EVGPVTMAGL INLSSTPTNR MIRYDYATKT GQCGGVLCAT GKIFGIHVGG NGRQGFSAQL KKQYFVEKQ

Protocol: 3C protease expression and purification — This document also contains some information on buffer components that affect protease activity.