Cryo-EM Links & Resources
Below are links to serve as a starting points for EM and the Cryo-EM world. Don’t hesitate to Contact Us with questions.
Access Internal Documents & Resources here, VUNetID protected
Set up an account for ACCRE and SBGrid here, VUNetID protected
General Information
Collaborative Computational Project for Electron Cryo-Microscopy – Medial Research Council
Getting Started in Cryo-EM – Lecture videos from Caltech
Beginners Guide to TEM – by John Rodenburg
CryoEM101 – Workflow tutorial from University of Utah
Cryo-EM Principles – Yale University, lectures and reading
MyScope TEM Introduction – Australian Microscopy & Microanalysis Research Facility
Cryo-ET and Subtomogram Averaging – Methods in Enzymology chapter by W. Wan & JAG Briggs
SBGridTV – Videos from events hosted by SBGrid on EM, crystallography, structure analysis, computational chemistry and more
Electron Cryo-Microscopy Course 2017 – Lectures from the MRC Laboratory of Molecular Biology
Official 2017 Nobel Lectures in Physics and Chemistry at Stockholm University – Lectures on the history and scientific accomplishments in the Cryo-EM field by Richard Henderson, Jacques Dubochet, and Joachim Frank
SmarterEveryDay, Episode 300: Featuring the Iverson lab’s bacterial flagellar motor & a tour of the CSB CryoEM Facility
Tutorials & Techniques
Visualizing Proteins and Macromolecular Complexes by Negative Stain EM – Jove video
Graphene Oxide Grid Preparation – by Thomas G. Martin, Scheres Group, Laboratory of Molecular Biology, Cambridge, UK
EM-University – Workflow videos for using Vitrobot, clipping grids, and EPU
RELION – Wiki & tutorial
Software Tools for Molecular Microscopy – WikiBooks
Tomography Software – NoskeWiki
Electron Microscopy – YouTube playlist by SBGrid Consortium
Cryo-EM Images & Common Features Gallery – CryoEM101, University of Utah
Electron Dose Rate Calculator – SubAngstrom
Cryo Sample Prep Complete Workflow: Vitrobot (2-26 min), AutoGrid Clipping (26-36 min), Cassette Loading & Docking (36-43 min) – Caltech
Meetings & Workshops
3DEM meetings and workshops – EMDataResource
Simons Electron Microscopy Center Workshops and Courses – New York Structural Biology Center
Workshops and Symposia at Outside Organizations – New York Structural Biology Center
Databases
Unified Data Source for 3-Dimensional Electron Microscopy – EMDataBank
Electron Microscopy Data Bank – Protein Data Bank in Europe
Electron Microscopy Public Image Archive – public resource for raw, 2D electron microscopy images
Mailing Lists
3DEM – Information for the 3DEM Community
CCPEM – Email discussion lists for the UK Education and Research Communities